|Muhammad Shafiqul Hoq Akand, Nizamul Haque Patwary, Zuairia Sultana, Mst. Mahbuba Khatun and Ruhal Amin; Pages: 103-107
Muhammad Shafiqul Hoq Akand1, Nizamul Haque Patwary1, Zuairia Sultana1, Mst. Mahbuba Khatun1, Ruhal Amin2
1Department of Agricultural Extension, Khamarbari, Dhaka-1215, Bangladesh
The present experiment was conducted at the Horticulture farm of Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, to study the effect of growth regulators on the growth and flowering of tuberose during the period from 01 May 2005 to 30 April 2006. Three different treatments of growth regulators (GA3, Ethrel and control) as foliar application were used for this purpose. Data were collected on flower yield and yield contributing character. The results demonstrated that foliar application of growth regulators had significant effect on the parameters studied except number of floret in 100 gm. GA3 showed better performance in respect of plant height but ethrel slightly reduced plant height compared to the control. GA3 and ethrel showed better performance in case of number of leaves per plant over control. Growth regulators influenced number of side shoot/plant. Maximum number of side shoot per plant 16.61 was obtained from GA3, while the least number of side shoot per plant (12.19) was found in control. The highest height of the side shoot was found 53.86 cm from GA3 while lowest height of side shoot 49.00 cm was found from ethrel tretment. The maximum number of leaves 14.96 was obtained from the plant treated with GA3 which is the least in control (12.15). The plants sprayed with GA3 initiated spike earlier (77.5 days) but it was 82.56 days in control. The maximum duration of flowering (18.13 days) was obtained from plants treated with ethrel while minimum duration of flowering (15.55 days) was found in the control. Length of the spike both for mother plant and for side shoot, including length of rachis increased with the increase of the bulb size and application of GA3. GA3 gave the highest spike diameter (0.99 cm) while minimum spike diameter (0.86) was obtained from the control. GA3 gave the maximum weight of single spike (39.00 gm) but control treatment (35.66 gm) was found to be inferior to the all other treatments. GA3 produced the maximum number of florets per spike (43.32) while the minimum number of floret per spike (38.91) was found from control. GA3 produced the highest number (380.59 thousands) of spike/ha while minimum number of spikes/ha (339.05 thousands) was in control treatment. The highest yield (15.00 t/ha) was obtained from the treatment GA3 but the control plants produced the lowest yield of spike per hectare (12.40 ton/ha).
Key words: Growth regulator, Growth performance, Flowering, Tuberose
Cite this article: Akand M.S.H., Patwary N.H., Sultana Z., Khatun M.M. and Amin R. (2016). Effect of growth regulators on growth and flowering of tuberose cv. single. Intl. J. Appl. Res. 2, 3, 103-107.
|Shahana Sultana and Md. Shahiduzzaman; Pages: 108-112
Shahana Sultana* and Md. Shahiduzzaman
On-Farm Research Division, Bangladesh Agricultural Research Institute (BARI), Mymensingh-2200, Bangladesh
The experiment was conducted at Muktagacha, Mymensingh during 2015-16, to study the effect of three different plant spacing on the growth and yield of sweet potato varieties aimed at designing a production system for medium sized sweet potato tubers. Three plant spacing viz. 60 cm × 30 cm, 50 cm × 30 cm and 30 cm × 25 cm (farmers’ practice) were used as treatment variables. BARI SP 11, an improved sweet potato variety was compared with a local sweet potato cultivar through this experiment. Results revealed that 50 cm × 30 cm plant spacing performed better in case of number of tuber per plant, tuber weight, tuber diameter and yielded the highest (20.53 t ha-1) followed by 60 cm × 30 cm spacing (17.07 t ha-1). The lowest tuber per plant, tuber weight, tuber diameter and tuber yield (15.22 t ha-1) was recorded with 30 cm × 25 cm plant spacing. Between the two varieties, BARI SP 11 performed better in all the parameters (vine length, tuber weight per plant, tuber length and diameter) except numbers of tuber per plant and yielded highest (18.29 t ha-1) over local cultivar (16.92 t ha-1). However, there were no significant different in case of interactions between plant spacing and sweet potato varieties. Economic analysis showed that the spacing 50 cm × 30 cm along with BARI SP 11 gave the maximum gross return (Tk 2,53,560 ha-1) and gross margin (Tk. 1,88,060 ha-1). The lowest gross return (Tk 1,80,000 ha-1) and gross margin (Tk 1,08,610 ha-1) was estimated from local sweet potato with farmers’ practice (30 cm × 25 cm). The highest BCR (3.87) was found from BARI SP 11 with 50 cm × 30 cm spacing and the lowest BCR (2.52) was calculated from local cultivar with 30 cm × 25 cm spacing. Therefore, 50 cm × 30 cm spacing are recommended for the cultivation of sweet potato for both the varieties in Muktagacha of Mymensingh district.
Key words: Sweet potato, Spacing, Cultivar, Vine, Tuber yield
Cite this article: Sultana S. and Shahiduzzaman M. (2016). Effect of plant spacing on the growth and yield of sweet potato cultivars in medium highland condition under AEZ-9. Intl. J. Appl. Res. 2, 3, 108-112.
|Md. Hadiujjaman, Md. Mostafizur Rahman, Md. Didarul Ahasan, Most. Arjuman Banu, Mst. Minara Khatun and Md. Alimul Islam; Pages: 113-121
Md. Hadiujjaman1,5, Md. Mostafizur Rahman2*, Md. Didarul Ahasan3, Most. Arjuman Banu4, Mst. Minara Khatun5, Md. Alimul Islam5
1Upazila Livestock Officer, Department of Livestock Services, Fulchhari, Gaibandha-6760, Bangladesh
Escherichia Coli isolated from broiler, layer, sonali and indigenous breeds of chicken from Bogra, Gazipur and Joypurhat districts of Bangladesh were used for in-vitro drug sensitivity test in the Department of Microbiology and Hygiene, Bangladesh Agricultural University. Eight commonly used antibiotic discs of different groups were used for antibiotic sensitivity tests of the isolated E. coli. Among the antibiotics Amoxicillin and Tetracycline were 100% resistant and 0% sensitive and intermediate sensitive. Ciprofloxacin was 4.48% sensitive, 20.90% intermediate and 74% resistant. Cephradin was 0% sensitive, 20.90% intermediate and 79.10% resistant. Cephalexin was 20.90% sensitive, 55.22% intermediate and 23.88% resistant. Gentamycin was 89.55%, 4.48% intermediate to 7.46% resistant. Kanamycin was 86.57% sensitive, 5.97% intermediate and 7.46 % resistant. Streptomycin was 1.49% sensitive, 0% intermediate and 80.60% resistant. Considering all the matters multidrug resistant E. coli is prevailing in apparently healthy chicken, which may become a big threat for poultry industry and as well as for human health.
Key words: Antibiotic sensitivity, E. coli, Healthy chicken, Bangladesh
Cite this article: Hadiujjaman M., Rahman M.M., Ahasan M.D., Banu M. A., Khatun M. M. and Islam M. A. (2016). Antibiotic sensitivity of Escherichia Coli isolated from apparently healthy chicken of selected areas of Bangladesh. Intl. J. Appl. Res. 2, 3, 113-121.
|K Samaddar, MM Rahman, Z Haque, MM Haq, MA Rahman, MR Molla ; Pages: 122-126
K Samaddar1, MM Rahman*, Z Haque2, MM Haq1, MA Rahman1, MR Molla1
1Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, Bangladesh
The reproductive disorders are major causes of reduced fertility in cows that diminish the total annual calf crop resulting in great economic loss in Bangladesh. The aim of the study was to find out the relative prevalence of reproductive disorders in crossbred and indigenous cows and goat in the South-Western Bangladesh. Records of reproductive disorders were reviewed from the official disease register of Upazilla (Sub-district) Livestock Offices during April 2014 to March 2015. Seasonal occurrence of reproductive disorders was recorded. Descriptive statistics was done to calculate the prevalence of different reproductive disorders of cattle in different regions of the Mymensingh district. Out of 76,601 clinical cases, a total of 3369 reproductive disorders of cows and does were screened out as study population. The overall prevalence of reproductive disorders was 4.4% in the South-Western Bangladesh. The occurance of reproductive disorders was higher in Jessore (32.88%) and lower in Bagerhat (9.08%). The study reveals that the most common reproductive surgical affections in the study area are Teat crack&Teat obstruction (28.3%) followed by Retained Placenta (23.3%), Vaginal prolapsed(16.8), Uterine prolapsed (10.3%), Gangrenous mastitis (8.9%), Vginal myiasis (6.9%), Pyometra (2.5%) and Dystocia (2.3%). Prevalence of Surgical reproductive disorders was recorded higher in Cows (66.13%) than in Does (33.86%) in The South-Western Bangladesh. The prevalence of reproductive disorders was higher in crossbreed in both cattle (44.43%) and goat (22.05%) than that of indigenous cattle (21.67%) and goat (11.84%). Moreover, In case of Cows, occurrence of surgical disorders was higher (45.80%) in the summer season followed by the winter (12.97%) and the rainy season (7.36%) Likewise the percentage of surgical disorders was higher in Summer (21.28%) than that of Rainy (2.43%) and Winter Season (2.05%) in case of goat. It seems that all these reproductive surgical affections may leads to severe health hazards to cattle and goat and the constraint on reproduction of the animals in Bangladesh and thus economical losses occurred. Reproductive health care practice needs to be strengthened for good economic return. This study also suggests that proper surgical correction by skilled veterinarian could solve the problem as early as possible in order to protect the animal from these maladies.
Key words: Surgical affection, Reproduction, Cow and Doe, South-West Bangladesh
Cite this article: Samaddar K., Rahman M.M., Haque Z., Haq M.M., Rahman M.A. and Molla M.R. (2016). Surgical affections of reproductive disorders in cows and does in the south-western Bangladesh. Intl. J. Appl. Res. 2, 3, 122-126.
|Rumana Momotaz, Most Arifunnahar, Shaikh Sharmin Siddique, Salma Sarker and Remi Chakma; Pages: 127-133
Rumana Momotaz1,*, Most Arifunnahar1, Shaikh Sharmin Siddique3, Salma Sarker1 and Remi Chakma2
1Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh
Sclerotium rolfsii demonstrated major harmful effect on plant growth due to strict root colonization, whereas, presence of the microbial antagonists showed significant optimistic effect on plant growth by declining the colonization of roots by S. rolfsii. The efficacy of seed treatment, soil treated with Tricho compost and soil drenching with fungicide on foot rot of groundnut was studied in the research field and laboratory of Bangladesh Agricultural Research Institute, Bangladesh. The experiment was designed as a Randomized Completely Block Design (RCBD) containing eight treatments. All treatments (1. soil drenching by Contaf 5 EC 0.1 %, 2. seed treatment with Provex 2.5 gkg-1 seed, 3. soil treatment by Tricho compost 2.5 tha-1, 4. combine application of soil treatment by Contaf 5 EC 0.1 % and seed treatment with Provex 2.5 gkg-1 seed, 5. soil drenching by Contaf 5 EC 0.1 % and soil treated by Tricho compost 2.5 tha-1, 6. seed treatment with Provex 2.5 gkg-1 seed and soil treated by Tricho compost 2.5 tha-1, and 7. soil drenching by Contaf 5 EC 0.1 %, seed treatment with Provex 2.5 gkg-1 seed and soil treated by Tricho compost 2.5 tha-1) tested confirmed significantly lower percentages of foot rot infection by Sclerotium rolfsii and resulted in higher nut yield compared to untreated control. The highest nut yield was observed in case of seed treatment with Provex 2.5 gkg-1 seed and soil drenching with Contaf 5 EC 0.1 %, followed by another combination of seed treatment, soil drenching and soil treatment. Although, individual application of different treatments did not gave as better results as combine application of treatments. Our findings pointed out the effective management of foot rot and significant yield benefit from groundnut can be obtained through integrated use of seed treatment with Provex 2.5 gkg-1 seed and Contaf 5 EC 0.1 % for soil drenching.
Key words: Foot rot, Groundnut, Trichoderma, Integrated Disease Management, Sclerotium rolfsii
Cite this article: Momotaz, R., Arifunnaha, M., Siddique, S.S., Sarker, S. and Chakma, R. (2016). Integrated management of foot rot disease of groundnut with seed and soil treatments. Intl. J. Appl. Res. 2, 3, 127-133.
|Nazmunnahar Nipa, Md. Rais Uddin Mian and Md. Aynul Haque; Pages: 134-140
Nazmunnahar Nipa, Md. Rais Uddin Mian* and Md. Aynul Haque
Department of Agricultural Finance, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
The present study was undertaken to observe the socioeconomic status of Tangail Saree (women cloth) handloom weavers and their problems. Keeping in view the objective, 50 respondents were selected from 5 villages of Tangail sadar upazila of whom 28 were small, 13 were medium and those of 9 were large according to loan size taken from various sources. Socio-economic characteristics of the weavers revealed that majority of the respondents were in 20-40 years. The overall family size stood at 5.42. All the weavers were literate, however only 22 % were qualified above secondary level. Almost 82 % respondents were engaged in weaving as their main occupation and some of them had other occupations as subsidiary. The average annual incomes of the respondents were Tk 164050 for small, Tk 294615.38 for medium and Tk 730311.11 for large category. The respective average annual expenditures were Tk 157850, 285384.62 and 16533.33. That’s why the annual average savings of the weavers were Tk 6529, 9230.78 and 13777.78 respectively. The study identified some problems like lack of adequate credit facilities, high price of raw materials, low price of product, political viability, lack of proper technology, etc. The respondents also suggested some solutions from their own point of view. Provision of institutional credit, increased marketing facilities, govt. patronage, etc. might help the respondents to get rid of the identified problems. .
Key words: Handloom weaver, Socioeconomic status, Problem, Tangail Saree
Cite this article: Nipa, N., Mian, M. R. U. and Haque, M. A. (2016). Socioeconomic status and problem of Tangail Saree handloom weavers in Bangladesh. Intl. J. Appl. Res. 2, 3, 134-140.
|Jubaidur Rahman, Mukaddasul Islam Riad, Fouzia Sultana Shikha, Md. Faridul Islam and Nasima Akter; Pages: 141-144
Jubaidur Rahman1*, Mukaddasul Islam Riad2, Fouzia Sultana Shikha3, Md. Faridul Islam1 and Nasima Akter4
1Agronomy Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh
An Experiment was conducted at the Regional Agricultural Research Station, Jamalpur during rabi 2014-2015 and rabi 2015-2016 to find out the optimum plant population of brinjal in brinjal sweet gourd intercropping systems(s). Intercrop combinations were viz., sole brinjal (80 cm × 60 cm), sole sweet gourd (2 m × 2 m), 100% brinjal + 100% sweet gourd, 100% brinjal + 75% sweet gourd, 100% brinjal + 50% sweet gourd and 100% brinjal + 25% sweet gourd. The result indicated that the highest gross return (Tk. 599300/ha) and cost of cultivation (Tk. 140000/ha) were obtained from (2014-15 and 2015-16) 100% brinjal + 100% sweet gourd followed by 100% brinjal + 75% sweet gourd. The highest gross margin (Tk 462200/ha) and BCR (4.37) were obtained from (2014-15 and 2015-16) 100% brinjal + 75% sweet gourd followed by 100% brinjal + 100% sweet gourd. Brinjal equivalent yield of all intercropping was higher than sole cropping indicating higher productivity of intercropping. The highest brinjal equivalent yield (2014-15 and 2015-16) (29.97 and 28.65 t/ha) was found in followed by 100% brinjal + 100% sweet gourd followed by 100% brinjal + 75% sweet gourd. 100% brinjal + 75% sweet gourd and 100% brinjal + 100% sweet gourd would be agronomically feasible and economically profitable.
Key words: Intercropping, Sweet gourd, Brinjal, Benefit cost ratio
Cite this article: Rahman, J., Riad, M.I., Shikha, F.S., Islam, M.F. and Akter, N. (2016). Intercropping of sweet gourd with brinjal at different plant population. Intl. J. Appl. Res. 2, 3, 141-144.
|Mst. Shamsun Naher, Md. Abdul Wadud and Abu Hena Faisal Fahim; Pages: 145-150
Mst. Shamsun Naher*, Md. Abdul Wadud and Abu Hena Faisal Fahim
Spices Research Centre, Shibganj, Bogra, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh
A field experiment was carried out at Sher-e-Bangla Agricultural University Farm during the kharif period from July, 2011 to November, 2011 to evaluate the effect of integrated nutrient management on growth and yield of T. Aman rice. The experiment comprised of eleven inorganic and organic manure combination viz., T0 (Control), T1 (N120 P15K45S20, Recommended dose), T2 (80% NPKS + 2 t ha-1 DH), T3 (80% NPKS + 4 t ha-1 CD), T4 (80% NPKS +1 t ha-1 DH + 2 t ha-1 CD),T5 (70% NPKS + 4 t ha-1 DH),T6 (70% NPKS + 8 t ha-1 CD), T7 (70% NPKS + 2 t ha-1 DH + 4 t ha-1 CD), T8 (50% NPKS + 6 t ha-1 DH), T9 (50% NPKS + 12 t ha-1 CD) and T10 (50% NPKS + 3 t ha-1 DH + 6 t ha-1 CD).. Application of 70% NPKS fertilizers + 4 t ha-1 GM produced the highest grain yield ( 5.9 t ha-1 ) and the second highest grain yield (5.85 t ha -1) was obtained from T2 (80% NPKS + 2 t ha-1 DH) treatment. Grain yield of T. Aman rice was positively correlated with number of effective tiller, plant height, panicle length, filled grain per panicle and straw yield. In straw yield, the treatment T3 (80% NPKS + 4 t ha-1 CD) produced the highest yield (8.59 t ha-1) and the second highest straw yield (8.08 t ha-1) was obtained from T1 (N120 P15 K45 S20, Recommended dose). The grain yield increases over control and ranges between 31.18 to 86.31 %. Therefore combined application of 70% NPKS fertilizers with 4 t/ha DH would results in higher number of effective tiller, plant height, panicle length and filled grains panicle-1 and 1000 grain weight. BRRI dhan40 at the Tejgoan silty loam soil.
Key words: Inorganic fertilizer, Cowdung, Green manure, Dhaincha, Silty loam soil
Cite this article: Nahar, M.S., Wadud, M.A., Fahim, A.H.F. (2016). Integrated nutrient management affect growth and yield of T. aman rice. Intl. J. Appl. Res. 2, 3, 145-150.
|Fouzia Sultana Shikha, Nargis Sultana, Md. Arifur Rahman, Sariful Haque Bhuiya, Jubaidur Rahman and Nasima Akter; Pages: 151-156
Fouzia Sultana Shikha1,*, Nargis Sultana2, Md. Arifur Rahman1, Sariful Haque Bhuiya3, Jubaidur Rahman4 and Nasima Akter2
1Soil Science Division, Regional Agricultural Research Station, Jamalpur-2000, Bangladesh
Optimum dose of potassium is essential for successful carrot production in different soil structures of Bangladesh. In this experiment, carrot was cultivated in silt clay loam soils at Regional Agricultural Research Station under Jamalpur district of Bangladesh during winter season of 2013-2014 and 2014-2015. The objective was to find out an appropriate rate of potassium for higher yield and quality of carrot. There were seven fertilizer combinations comprising T1: Recommended dose (100-30-12-2.5 kg N-P-S-Zn ha-1 with 1.5 ton cowdung ha-1) + 0 kg K ha-1, T2: RD + 30 kg K ha-1, T3: RD + 50 kg K ha-1, T4: RD + 70 kg K ha-1, T5: RD + 90 kg K ha-1, T6: RD + 110 kg K ha-1 and T7: Farmer’s practice (60-90-70 kg N-P-K ha-1 with 1.0 ton CD ha-1). The favorable effects of K on the growth, total yield and root parameters were obtained when carrot plants fertilized with 70 kg K ha-1 plus recommended dose of other fertilizers. Carrot yield and quality was increased gradually with K fertilization and highest yield was recorded from 70 kg K ha-1, however statistically similar yield was obtained from 50, 90 and 110 kg K ha-1 in addition to recommended dose of other fertilizers. The estimated optimum and economic rate of K from the simple polynomial regression equation were 92 kg K ha-1 and 91 kg K ha-1, respectively.
Key words: Daucus carota L., Potassium requirement, Carrot root yield, Response curve, Moisture content
Cite this article: Shikha, F.A., Sultana, N., Rahman, M.A., Bhuiya, S.H., Rahman, J., Akter, N. (2016). Effect of potassium fertilization on the growth, yield and root quality of carrot. Intl. J. Appl. Res. 2, 3, 151-156.
|Md. Faridul Islam, Sadikun Nahar, Jubaidur Rahman, Md. Parvez Anwar and Md. Anowerul Islam; Pages: 157-163
Md. Faridul Islam1*, Sadikun Nahar2, Jubaidur Rahman3, Md. Parvez Anwar2 and Md. Anowerul Islam4
1Agronomy Division, Regional Agricultural Research Station, Akbarpur, Moulvibazar-3210, Bangladesh
A field experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh to evaluate the effect of integrated nutrient management on the performance of three transplant Aman rice varieties. The treatments included were three varieties of transplant Aman rice viz. BRRI dhan31, BRRI dhan39 and BRRI dhan41 and five different integrated nutrient managements viz. recommended dose of chemical fertilizers (RF), Cowdung @ 10 t ha-1, 50% RF + Cowdung @ 5 t ha-1, 75% RF + Cowdung @ 2.5 t ha-1 and 100% RF + Cowdung @ 5 t ha-1. The experiment was laid out in a randomized complete block design with three replications. It was found that variety had significant effect on all the crop characters except panicle length and 1000-grain weight. Among the three varieties of transplant Aman rice, BRRI dhan31 produced the highest grain yield and BRRI dhan39 produced the lowest grain yield. All the characters were significantly influenced by different integrated nutrient managements except panicle length and 1000-grain weight. The highest grain yield was obtained from 50% RF + Cowdung @ 5 t ha-1 and the lowest grain yield was received from RF. Interaction effect of variety and integrated nutrient management was significant on all crop characters except panicle length and 1000-grain weight. The highest grain yield was obtained from BRRI dhan31 with 50% RF + Cowdung @ 5 t ha-1. The same variety with Cowdung @10 tha-1 also produced identical grain yield. The performance of 50% RF + Cowdung @ 5 t ha-1 was found to be superior to any other nutrient management and BRRI dhan31 was found to be the best among the varieties. Therefore, BRRI dhan31 with 50% RF + Cowdung @ 5 t ha-1 can be recommended for obtaining higher grain yield.
Key words: Manures and fertilizers, Nitrogenous fertilizer, Grain yield, Biological yield, Varietal difference, Transplant aman rice
Cite this article: Islam, M.F., Nahar, S., Rahman, J., Anwar, M.P., & Islam, M.A. (2016). Cowdung based integrated nutrient management for transplant Aman rice varieties. Intl. J. Appl. Res. 2, 3, 157-163.
|S. Nasrin, M.H. Kabir, M.A. Hossain, M.M. Hossain and M.A. Hashem; Pages: 164-171
S. Nasrin1, M.H. Kabir2, M.A. Hossain1, M.M. Hossain1 and M.A. Hashem1*
1Department of Animal Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
The study was carried out to determine appropriate level of wheat flour with bottle gourd leaves extract which can be used in meatball production. Four treatment groups treated as control group (T1), (T2– 5% bottle gourd leaves extract + 5% wheat flour), T3 (5% bottle gourd leaves extract+ 8% wheat flour ) and T4 (5% bottle gourd leaves extract +10% wheat flour) respectively. Meatballs were refrigerated under an aerobic condition. Different analysis like proximate, sensory, physicochemical, biochemical and microbiological analyses were used for quality assessment. DM matter content was increased with storage period. Color, cooking loss, flavor, tenderness and overall acceptability were differed significantly at different treatment levels (p<0.05). Crude protein, ether extract, raw and cooked pH were decreased significantly (p<0.05) with storage period. TBRAS value, total viable count, total coli form count and yeast-mould count of meatballs were differed significantly (p< 0.05) at different levels. T2 was more acceptable than other treatment groups contain 5% bottle gourd leaves extract and 5% wheat flour. It may be concluded that wheat flour as prebiotics and bottle gourd leaves extract as antioxidant and antimicrobial can be used in manufacture of meatball successfully.
Key words: Value added, Meatballs, Bottle gourd leaf extract, Wheat flour
Cite this article: Nasrin, S., Kabir, M.H., Hossain, M.A., Hossain, M.M. & Hashem, M.A. (2016). Formulation of value added beef meatballs using bottle gourd (Lagenaria siceraria) leaf extracts and wheat flour. Intl. J. Appl. Res. 2, 3, 164-171.
|M.A. Khaleque, F. Boby, M. Shahiduzzaman; Pages: 172-176
M.A. Khaleque, F. Boby, M. Shahiduzzaman*
Department of Parasitology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
The aim of this study was molecular detection and characterization of Cryptosporidium isolated from animal and human stool sample. A polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique based on the small-subunit rRNA gene was used for the analysis of 10 positive samples (9 samples from human and 1 sample from animal). Nine human samples were collected from Shurjo Kanti (S.K.) Hospital, Mymensingh that has a diarrheal disease section and 1 animal sample was collected from Bangladesh Agricultural University campus. The samples were employed for DNA extraction using Purelink™ Genomic DNA Mini Kit (Invitrogen, USA) and then PCR was performed. Five PCR positive Cryptosporidium DNA were genotyped by using VspI (AseI) restriction enzyme (Lithuania, EU). Cryptosporidium parvum, Bovine A gene was detected in 2 samples, Cryptosporidium parvum, Bovine B gene was detected in 1 sample and Cryptosporidium hominis was detected in 2 samples. The present study confirms the Cryptosporidium genotype (Cryptosporidium parvum, Bovine A gene, Cryptosporidium parvum, Bovine B gene and Cryptosporidium hominis) in the patient admitted to Shurjo Kanti (S.K.) Hospital, Mymensingh, indicating the existence of Cryptosporidium genotyes in the study areas. However, further studies are needed to better understanding the transmission dynamics of these genotypes and thereafter taking necessary measure to control and/or prevent the disease.
Key words: Cryptosporidium, human, child, animal, Bangladesh
Cite this article: Khaleque, M.A., Boby, F. & Shahiduzzaman, M. (2016). Molecular characterization of Cryptosporidium isolated from animal and human. Intl. J. Appl. Res. 2, 3, 172-176.